Availability: OCTOBER 2006

A New Version for Topology Analyses (TOPO)

This version allows to do manual topology analyses with methods similar to those of WinRHIZO Pro (done automatically in the latter). It is available as an option to WinRHIZO Tron and WinRHIZO Tron MF. You can define up to two descending links (root segments) from each link and specify their hierarchy from which the following information can be extracted;

• Topological link classification into five categories: EE (External-External), EI (External-Internal), (Internal-Internal), IL (Isolated Link), BL (Base Link).
  
• Branching angle (angle between a descending link direction and the direction of the link it is issued from).
  
• Link magnitude (number of external links [with a tip] extending from a link.
  
• Link path length (number of links between a link and the base link).
  
• Link developmental order (primary, secondary, tertiary order...).
  
• Root system altitude (largest path length)
  
• Root system external path length (sum of path lengths of external links (tip).
  
• Axis morphology (length, area, volume of connected links of the same order).
  
  It is possible to display some of the above classifications over the links in the image using different color schemes.

New features of all versions

• The Roots/Continue Root command has been renamed Add Descending Segment. This command can be used to prolong an existing root or (for the TOPO version) to make a fork and add a second descending link (brother) to a segment which has already one).
• The Root/Delete Segment command has been renamed Root/Delete Branch which can now be used to delete a single segment or a segment and all its descendants.
  
• Roots can now rollover from one image side to the other (once a root is shifted passed the image boundary it is displayed on the opposite side as if the image was continuous. This feature is for images that come from scanners which scan a 360 field of view in tubes.
  
• Tips can be invalidated when the root ending in an image is not the real root end, such as when a segment continues on another image. A symbol (+) is now drawn at each valid tip.
• There are more image magnifications (zoom factors) and a fit to screen option.
• The data file format has been modified to accommodate the new data. XLRhizo Tron 2006a is required to analyse files made by WinRHIZO Tron 2006a.
• It is now simpler and faster to calibrate and analyse an image with roots and a calibration target in the same image. WinRHZO TRON will make the calibration on the displayed image (rather than loading another image).
• There are now 14 information colors that can be changed instead of 12 in Display/Information Colors.
• You can specify the RGB values of information colors with numbers from 0 to 255 (in addition to moving the scroll bars).
• The "Root properties" and "Segment properties" window buttons text is changed when these commands are activated from the menus to validate/edit a root or segment properties (as opposed to when they are displayed during root creation) to display more appropriate actions (Ok continue is replaced by Ok, Stop is hidden and Cancel node is replaced by Cancel).
• "Drawing style" paths are no more available.

Bug fixes

• When continuing a root, the previously active segment is deactivated (the problem happened only when the active segment was not the last of the root).

Availability: March 2005

• Up to four user-definable observations (such as fungae presence, root color...) can be specified for each image (frame), root or root segment. These observations and can be set even if there are no root in the image. They replace the former user variable.
• You can display a single color channel (Red, Green or Blue) of an RGB color image. Sometimes the root-background contrasts are higher in one color channel.
• The global data lines now include the total root length, area, volume, number of tips and average diameter for the dead and gone categories of roots/segments (in addition to those that were present before [alive, alive+dead+gone]).
• Calibration of images from a camera can be done with a single mouse click when Regent's new calibration targets are included in the image.
• Text size is medium size by default instead of small.
• Scrollbar thumb works with larger images.
• The appearance of most windows (dialogs to enter settings) have been redesigned.
• Camera has been merged with Scanner in Image/Origin.
• Image acquisition via TWAIN compatible devices is now possible.
• Flickering when resizing the main window has been removed.
• About command has been moved to Help menu.
• Compatible with XLRhizotron 2005a.
• Fixed bugs
  • The image dpi is no more asked when analysing images from scanners with the Intrinsic calibration method.
  • The right date and time are displayed on screen when loading more than one session (tube, tile).

Availability: 24 January 2003

This version solves a bug of version 2003b in root distribution data in function of diameter when the diameter classes were not equal (when Bohm or Custom classification was used).

Availability: 06 January 2003

This version is between 100% to 1000% faster when loading multiple frames and it uses a lot less memory.
Tron will now automatically set two new root variables. These are:
Birth session: the session number that the root has been interactively created by the operator.
Death session: the session number that the root has been declared dead or gone by the operator.

WinRHIZO Tron & Tron MF 2003a features:

Availability: December 2002

• Root surface area, projected area and volume per diameter class is now more precise. While the earlier versions used the class average diameter to calculate the above morphological parameters, the new version uses the exact diameter (and length) of each root sub-segment in each diameter classes.
• Irrelevant warning messages and windows have been removed from the calibration procedure.
• The maximum number of roots that can be analysed per region and the maximum number of regions have been increased by an order of magnitude.
• Analysis/Root Creation Parameters has been renamed Analysis/Parameters.
• If you load an image while no calibration has been done, the loading process is aborted resulting in the suppression of some useless messages.
• A command that allows to display only root growth has been added. Earlier versions had a command to highlight growth, the new one can display root growth exclusively by hiding root segments that were not affected in time.
• The Root Properties window displayed during root tracing has one more button that allows to retain the last node created and stop the tracing process (instead of continuing it).
• The T-shaped cursor displayed over the image during root creation is more visible (it is in color rather than grey).
• The color of the text in the command area and the information displayed over the image (roots and regions names, root edges...) can be changed.
• The manual has been updated and improved.
• It has a new icon different from the WinRHIZO Basic, Reg and Pro icon.
• The automatic root number counter (of the automatic naming scheme) is automatically updated per region rather than for the image. The root number suggested by default is always the largest existing root number plus one for a given region.
• A useless warning message has been removed when the root distribution graphic horizontal scale is changed.
• Images scanned in dots per cm (instead of dots per inch) are analysed more precisely (a very rare situation as the Tron version is used almost exclusively to analyse images acquired with a camera).
• Navigation among frames, tiles or tubes via keyboard keys has been removed. You must click the acquisition icon or activate Image/Acquire Image.

Bugs solved

The Sample Identification window now works as expected when the operator modifies the default values displayed. When more than one image is loaded (slice, tile...) the information of this window cannot be edited, it can only be viewed.

The root surface area and projected area per diameter class of earlier version was 10 times too large (were in mm instead of cm) and volume 100 times too large. An e-mail had been sent to every earlier version owners to inform them of this problem.

Some bugs with tube loading have been removed. In some situations "load next" or "previous" tube didn't worked or used to crash when an image was missing.

Some bugs in "per session" analysis mode have been fixed (Tron used to switch to "per frame" occasionally.

Modifications has been brought to bmp files created with the Misc/Print commands so that they can be opened by more programs.

Root and segment properties of uneditable regions can no more be modified by clicking them in the image.

When roots are copied from a region and pasted to a different one, their session, location date and time are updated so that they become those of the region they are pasted to.

Shipping date: January 2003.

Features:

• A new XLRhizo has been done and renamed XLRhizoTron. You must use XLRhizoTron V. 2003b or later to analyse files produced by WinRHIZO Tron 2003b.

Known bugs: None

Compatibility: Can analyse files produced by WinRHIZO Tron & Tron MF

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