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The WinRHIZO program is offered in 4 versions (Basic, Reg, Pro & Arabidopsis)
The tables below list and explain the measurements and features offered by each version

WinRHIZO Basic is a low cost entry level version. It produces only global measurements (average root diameter, total root length, area, volume and number of tips).

WinRHIZO Regular has all features of the Basic model plus root morphology measurement as a function of user definable diameter classes. A root (length, area, volume) distribution graphic is automatically generated and is visible above the image. This graphic can also be printed.

WinRHIZO Pro has all features of the Regular version plus link, topology, architecture and color analyses. The color analysis can produce root morphology as a function of color. It can also be used as a color area meter (click here for details).

WinRHIZO Arabidopsis has all features of the Pro version plus the ability to separate and analyse seprately individual objects such as seedlings and leaves. It can do multiple root analyses per analysed image or regions of it (click here for details).

Measurements
Model
Root Morphology
Basic
Reg
Pro
Global (Total or Average for the image)
& Arabid.

Total Length

Yes
Yes
Yes

Average Diameter

Yes
Yes
Yes

Total Area, Volume

Yes
Yes
Yes

Number of tips, forks & crossings

Yes
Yes
Yes
In function of root diameter (per diameter class)
Length
No
Yes
Yes
Area, Volume
No
Yes
Yes
Number of tips
No
Yes
Yes
In function of root color (see Color Analysis below)
Length
No
No
Yes
Area, Volume
No
No
Yes
Number of tips
No
No
Yes
Note: It is possible, at any time, to upgrade from a lower to a higher program version by paying the difference in cost between the two versions at the time of the upgrade.
 
Link Analysis
Basic
Reg
Pro
 
& Arabid.
Globally (for te whole image)

Total number of links

No
No
Yes
Average link length, diameter, area, volume, branching angle
No
No
Yes
Per link (individually)
Lenght, Averiage Diameter, Area, Branching angle
No
No
Yes

Link analysis is a study of the morphology and basic connectivity of root segments. It can be done on incomplete or complete root systems.


Basic Connectivity (definitions & colors below refer to figure above)
Red
= External-External link
Yellow = External-Internal link
Green = Internal-Internal link
Blue = Isolated link (not connected to any link)

     
Topology
Basic
Reg
Pro
 
& Arabid.
Globally (for te whole image)

External path length

No
No
Yes
Altitude
No
No
Yes
Per link (individually)
Magnitude
No
No
Yes
Path length
No
No
Yes
Altitude
No
No
Yes
Structured Connectivity
No
No
Yes

Note: Topology and developmental analyses require an integral root system. It is meaningless if the connectivity of the root system’s links has been destroyed by manipulation or the imaging process. It is not recommended to do link analyses on dense root systems. If you cannot visually track root segments in an image, it is unlikely that RHIZO will be able to do so either. Measurements made on such images will not be precise and might contain errors.

Topology analysis is an extensive link connectivity analysis.

  • Magnitude, the number of external links extending from a link.
  • Path length, the number of links between a link and the base link, (inclusively).
  • External path length, the sum of path lengths of all external links. It is a value for the complete root system (not per link).
  • Altitude, the largest path length.
     
Developmental classification
Basic
Reg
Pro
 
& Arabid.

Number of links (per order)

No
No
Yes
Total length (per order)
No
No
Yes
Total area (per order)
No
No
Yes
Average link length (per order)
No
No
Yes
Average link area (per order)
No
No
Yes
Average diameter (per order)
No
No
Yes
Link order (per link)
No
No
Yes

Root Architecture with Fractals

No
No
Yes

Color Analysis (click here for details)

No
No
Yes

 

The developmental analysis identifies the order in which links we born from the base link as the plant grew.

Links of the same order can also be grouped per axis. An axis is a group of connected links of the same order. Morphological data can be obtained for all orders and axes.

in

Copyright (c) 1996-2009 by Regent Instruments Inc. for entire content. All rights reserved.

Modified October 29, 2009